Metabolic engineering is a scientific art. It requires intimate knowledge of metabolism and related substrates, products, and co-factors. Conagen’s scientists investigate the properties of various enzymes, their regulation and control mechanisms, and cellular and sub-cellular compartmentalization. Conagen’s engineers have extensive experience in fermentation scale-up, pilot plant operation, industrial process development, and world-scale production process design and improvement.
We have developed various technologies to successfully engineer many valuable molecules. Among these technologies are: ArchxyTeQTM for the construction of artificial enzyme complexes, FlexyPumpTM for the design and screening of specific cell membrane transporters, and QuixyScaleTM
for systematic optimization of high-throughput fermentation conditions.
This technology takes advantage of metabolic channeling inside multi-enzyme complexes to direct the flow of metabolites toward the specific sub-branch of a given pathway. By limiting the random diffusion of the intermediates, ArchxyTeQTM increases the catalytic efficiency of the entire pathway, boosting the accumulation of the final products while reducing side products.
By innovative adaptation of naturally occurring transport systems, FlexyPumpTM technology introduces compound-specific transporters into production microbes. Through targeted engineering and direct evolution, Conagen’s scientists can generate specific transporters to secrete the final product to the culture medium, improve culture conditions, and simplify the harvesting of the product compounds.
Identification of the best strains for scale-up and subsequent optimization of fermentation conditions are two time-consuming steps in industrial process development. QuixyScaleTM technology combines an automation system with micro-fluid fermenter technology to significantly reduce the lead-time in scale-up and optimization.